Human iPS Reprogramming Boost Supplement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Small molecules targeting specific signaling pathways have been shown to enhance the efficiency and/or replace the transcription factors required for reprogramming. EMD Millipore has screened multiple chemical compounds and selected the compounds in this kit based on their effects on enhancement of reprogramming efficiency as assessed by the number and overall quality and flat 2D morphology of human iPS colonies generated.

The Human iPS Reprogramming Boost Supplement contains two proprietary small molecules in amounts sufficient to supplement over 250 mL human ES/iPS cell maintenance medium. When used in conjunction with the Human STEMCCA lentivirus reprogramming kits (SCR544, SCR545), the Human iPS Reprogramming Boost Supplement enhanced the efficiency of human iPS colony formation over ten-fold and reduced the time to establish full reprogrammed colonies by nearly 50%. The colonies formed possessed the distinctive flat 2D morphology that are reminiscent of normal human ES cell colonies and could be easily passaged. Full reprogrammed human iPS colonies that are SSEA-4-positive, TRA-1-60-positive and Hoechst-Dim are readily established in approximately 20 – 25 days.

Kit contains sufficient quantities to supplement over 250 mL of medium.

1. TGF-ß RI Kinase Inhibitor VI Supplement (1000X): (Part No. CS204420) One (1) vial containing 300 µL of the inhibitor in high quality DMSO.

2. HDAC Inhibitor-A Supplement (1000X): (Part No. CS204423) One (1) vial containing 300 µL of the inhibitor in water.

Components of the Human iPS Reprogramming Boost Supplement are stable for at least 6 months from date of receipt when stored at -20°C. Upon first thaw, centrifuge the vial and gently mix the solution. Aliquot into smaller working volumes and freeze at -20°C or -80°C. Upon addition of the components of the Mouse iPS Reprogramming Boost Supplement to the media, filter the supplemented media with a 0.22 µM filtration unit. Supplemented media is good for up to two weeks when stored at 2-8°C.