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MAC506-100UG Mouse Anti-Llama IgG3 Antibody, clone 8E1

Mouse Anti-Llama IgG3, clone 8E1, Cat. No. MAC506, is a mouse monoclonal antibody that detects Llama IgG3 and is tested for use in ELISA, Immunoaffinity, Purification, and Western Blotting.

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MAC506-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAC506-100UG
      DescriptionMouse Anti-Llama IgG3 Antibody, clone 8E1
      Alternate Names
      • IgG3 HCAb
      • IgG3 Nanobody
      Background InformationLlama, camels, and alpacas are camelids that produce normal IgG1 like antibodies and also functional IgG isotypes that do not incorporate light chains and possess only heavy chains. These heavy-chain antibodies may constitute about 50% of the IgG in llama serum and as much as 75% of the IgG in camel serum. By virtue of their smaller size they exhibit better bio-distribution, tissue penetration, and monomeric behavior. IgG3 consists of dimers of short heavy chains, which are characterized by a normal Fc region without a CH1 domain. Although it lacks the CH1 region, it still retains an antigen binding domain called the VHH region. Clone 8E1 is shown to specifically bind to IgG3 and not to any of the IgG2 heavy chains. (Ref.: Daley, LP., et al. (2005). Clin. Diag. Lab. Immunol. 12(4); 380-386).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationMouse Anti-Llama IgG3, clone 8E1, Cat. No. MAC506, is a mouse monoclonal antibody that detects Llama IgG3 and is tested for use in ELISA, Immunoaffinity, Purification, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoaffinity Purification
      • ELISA
      Application NotesTested Applications

      Immunoaffinity Purification: A representative lot was used in Immunoaffinity Purification applications (Daley, L.P., et. al. (2005). Clin Diagn Lab Immunol. 12(3):380-6; Daley-Bauer, L.P., et. al. (2010). Clin Vaccine Immunol. 17(12):2007-15).

      ELISA Analysis: A representative lot detected Mouse Anti-Llama IgG3 in ELISA applications (Daley, L.P., et. al. (2005). Clin Diagn Lab Immunol. 12(3):380-6; Daley-Bauer, L.P., et. al. (2010). Clin Vaccine Immunol. 17(12):2007-15).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified Llama serum IgG3.
      EpitopeUnknown
      Clone8E1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 8E1 is a mouse monoclonal antibody that detects Llama IgG3.
      Species Reactivity
      • Mammals
      Species Reactivity NoteLlama.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with IgG3.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Llama IgG3, but not IgG1 and IgG2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAC506-100UG 04065266298437

      Documentation

      Mouse Anti-Llama IgG3 Antibody, clone 8E1 SDS

      Title

      Safety Data Sheet (SDS) 

      Mouse Anti-Llama IgG3 Antibody, clone 8E1 Certificates of Analysis

      TitleLot Number
      Mouse Anti-Llama IgG3, clone 8E1 - 3899617 3899617
      Mouse Anti-Llama IgG3, clone 8E1 - 3931791 3931791
      Mouse Anti-Llama IgG3, clone 8E1 - 3985009 3985009
      Mouse Anti-Llama IgG3, clone 8E1 - 4010114 4010114
      Mouse Anti-Llama IgG3, clone 8E1 - 4026474 4026474
      Mouse Anti-Llama IgG3, clone 8E1 - 4035509 4035509
      Mouse Anti-Llama IgG3, clone 8E1 - 4035834 4035834
      Mouse Anti-Llama IgG3, clone 8E1 - 4070802 4070802
      Mouse Anti-Llama IgG3, clone 8E1 - 4097754 4097754
      Mouse Anti-Llama IgG3, clone 8E1 - 4156827 4156827
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