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SCC049 O9-1 Mouse Cranial Neural Crest Cell Line

The O9-1 cell line stably expresses stem cell markers (CD44, Sca-1, and Bmi1) and neural crest markers (AP-2a, Twist1, Sox9, Myc, Ets1, Dlx1, Dlx2, Crabp1, Epha2, and Itgb1). O9-1 cells are capable of contributing to cranial mesenchymal (osteoblast and smooth muscle) neural crest fates.

O9-1 Mouse Cranial Neural Crest Cell Line MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture, Stem Cell Culture
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SCC049
≥1X10⁶ cells/vial  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      CULT, Cult
      Description
      Catalogue NumberSCC049
      DescriptionO9-1 Mouse Cranial Neural Crest Cell Line
      Background InformationCranial neural crest cells give rise to ectomesenchymal derivatives such as cranial bones, cartilage, smooth muscle, dentin, as well as melanocytes, corneal endothelial cells, and neurons and glial cells of the peripheral nervous system. Previous studies have suggested that although multipotent stem-like cells may exist during the course of cranial neural crest development, they are transient, undergoing lineage restriction early in embryonic development.

      Whole-genome expression profiling of O9-1 cells revealed that this line stably expresses stem cell markers (CD44, Sca-1, and Bmi1) and neural crest markers (AP-2a, Twist1, Sox9, Myc, Ets1, Dlx1, Dlx2, Crabp1, Epha2, and Itgb1). O9-1 cells are capable of contributing to cranial mesenchymal (osteoblast and smooth muscle) neural crest fates when injected into E13.5 mouse cranial tissue explants and chicken embryos. These results suggest that O9-1 cells represent multipotent mesenchymal cranial neural crest cells. The O9-1 cell line should serve as a useful tool for investigating the molecular properties of differentiating cranial neural crest cells. The O9-1 cell line can be propagated and passaged for at least 10 passages, and can differentiate into osteoblasts, chondrocytes, smooth muscle cells, and glial cells (Ishii, 2012).

      Ishii, M., et al. (2012) A stable cranial neural crest cell line from mouse. Stem Cells Dev. 21(17): 3069-3080.
      References
      Product Information
      Components
      • 1) ≥1X106 viable O9-1 Mouse Cranial Neural Crest Cell Line: (Catalog No. SCC049). Derived from mass cultures of Wnt1-Cre; R26R-GFP reporter-expressing cranial neuronal crest cells from E8.5 mouse embryos.
      PresentationProduct is supplied frozen in 10% DMSO and 90% expansion medium.
      Quality LevelMQ100
      Applications
      ApplicationThe O9-1 cell line stably expresses stem cell markers (CD44, Sca-1, and Bmi1) and neural crest markers (AP-2a, Twist1, Sox9, Myc, Ets1, Dlx1, Dlx2, Crabp1, Epha2, and Itgb1). O9-1 cells are capable of contributing to cranial mesenchymal (osteoblast and smooth muscle) neural crest fates.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Neural Lineage Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells at passage 20 -22
      • Cells tested negative for infectious disease by a murine PCR panel (Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services)
      • Cells tested negative for mycoplasma contamination
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsO9-1 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC049 04055977165920