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559309 PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb

This PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb is validated for use in WB, IHC for the detection of SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵).

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559309
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Nem, Ch, Dr, H, M, RRbPolyclonal Antibody

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      Description
      OverviewRecognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185.
    • Antibody Target Gene Symbol: MAPK8, MAPK9
    • Target Synonym: AI849689, C-JUN N-TERMINAL KINASE1, JNK, JNK1, JNK1 PROTEIN KINASE, JNK1A2, JNK21B1/2, p46JNK1, p46JNK1 ALPHA, PRKM8, Sapk gamma, SAPK P46, SAPK1, SAPK1/JNK, STRESS-ACTIVATED PROTEIN KINASE-LIKE KINASE, AI851083, Jnk p54, Jnk p55, JNK-55, JNK2, JNK2A, Jnk2a2, JNK2ALPHA, JNK2B, JNK2BETA, JUNKINASE, p54a, p54aSAPK, PRKM9, SAPK, SAPK ALPHA, SAPK ALPHA 1
    • Entrez Gene Name: mitogen-activated protein kinase 8
    • Hu Entrez ID: 5601
    • Mu Entrez ID: 26420
    • Rat Entrez ID: 50658
      		•
      Catalogue Number559309
      Brand Family Calbiochem®
      Application Data
      Detection of human JNK1 and JNK2, phosphorylated on Tyr183 and Thr185, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV irradiation (lanes 2-6). Primary antibody: PhosphoDetect™ Anti-SAPK/JNK (pTyr183,pThr185) Rabbit pAb (Cat. No. 559309) (1:1000) pre-incubated without peptide (lanes 1,6) or with immunogen phosphopeptide (lane 2), a generic phosphothreonine-containing peptide (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or non-phosphopeptide corresponding to the immunogen (lane 5). Detection: chemiluminescence.
      References
      ReferencesFanger, G.R., et al. 1999. Histol. Histopathol. 14, 887.
      Gabai, V.L., et al. 1998. FEBS Lett. 438, 1.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application CommentsSpecific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Nematode
      • Chicken
      • Drosophila
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      559309-10TCN 04055977192445

      Documentation

      PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-SAPK/JNK (pThr¹⁸³, Tyr¹⁸⁵) Rabbit pAb Certificates of Analysis

      TitleLot Number
      559309

      References

      Reference overview
      Fanger, G.R., et al. 1999. Histol. Histopathol. 14, 887.
      Gabai, V.L., et al. 1998. FEBS Lett. 438, 1.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-June-2009 JSW
      ApplicationImmunoblotting (1:1000)
      Immunohistochemistry (see comments)
      Application Data
      Detection of human JNK1 and JNK2, phosphorylated on Tyr183 and Thr185, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) or treated with UV irradiation (lanes 2-6). Primary antibody: PhosphoDetect™ Anti-SAPK/JNK (pTyr183,pThr185) Rabbit pAb (Cat. No. 559309) (1:1000) pre-incubated without peptide (lanes 1,6) or with immunogen phosphopeptide (lane 2), a generic phosphothreonine-containing peptide (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or non-phosphopeptide corresponding to the immunogen (lane 5). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr183 and Tyr185.
      BackgroundC-Jun-N-terminal kinase (JNK), also called stress-activated protein kinase (SAPK) is a member of the mitogen activated protein kinase (MAPK) family. JNK is activated in response to various extracellular stimuli, including pro-inflammatory cytokines. Phosphorylation of threonine and tyrosine in the Thr-Pro-Tyr domain is required for full activation. Mitogen activated protein kinase kinase (MKK4) preferentially phosphorylates tyrosine 185, and MKK7 causes preferential phosphorylation of threonine 183.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK
      IsotypeIgG
      SpeciesC. elegans, Drosophila, chicken, human, mouse, rat
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
      Preservative≤0.1% sodium azide
      CommentsSpecific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesFanger, G.R., et al. 1999. Histol. Histopathol. 14, 887.
      Gabai, V.L., et al. 1998. FEBS Lett. 438, 1.