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539251 Protein A, Alkaline Phosphatase Conjugate

This Protein A, Alkaline Phosphatase Conjugate is validated for use in Direct Immunoassay.

Protein A, Alkaline Phosphatase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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539251
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539251-1MGCN
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      Description
      OverviewNative protein A conjugated to alkaline phosphatase. Can be used instead of an alkaline phosphatase-conjugated secondary antibody in many immunological assays.
      Catalogue Number539251
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesDirect Immunoassay (1:5000)
      Application Commentsp-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      PuritySingle band by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      539251-1MGCN 04055977268980

      Documentation

      Protein A, Alkaline Phosphatase Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Protein A, Alkaline Phosphatase Conjugate Certificates of Analysis

      TitleLot Number
      539251
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2007 RFH
      ApplicationDirect Immunoassay (1:5000)
      DescriptionNative protein A from Staphylococcus aureus conjugated to alkaline phosphatase.
      BackgroundProtein A is the universal "second antibody" because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect, and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors, and therefore will not give high background reactions. Binding strength is as follows: strongly reactive: guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, Human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep.
      FormLiquid
      FormulationIn 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      PuritySingle band by SDS-PAGE
      Preservative≤0.1% sodium azide
      Commentsp-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling