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SCM009 Sigma-AldrichRat Neural Stem Cell Expansion Medium

The Rat Neural Stem Cell Expansion Medium is provided as a two-component system that is convenient & easy to use.

Rat Neural Stem Cell Expansion Medium MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Key Applications: Stem Cell Culture

SCM009

1 kit

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Overview

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Key Spec Table

Key Applications
Cult

Description
Catalogue Number	SCM009
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Rat Neural Stem Cell Expansion Medium
Overview	The Rat Neural Stem Cell Expansion Medium is provided as a two-component system that is convenient and easy to use. This product includes the Neural Stem Cell Basal Medium, a defined serum-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. A solution of basic FGF-2 growth factor is also provided separately, which when added to the Neural Stem Cell Basal Medium allows for the growth and proliferation of rat neural stem cells. Withdrawal of bFGF-2 from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of rat neural stem cells.
Materials Required but Not Delivered	• Cryopreserved Rat Cortical Neural Stem Cells (Millipore Catalog No. SCR022) • Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019) • Poly-L-ornithine (Sigma Catalog No. P3655) • Laminin (Millipore Catalog No. CC095) • Accutase solution (Millipore Catalog No. SCR005)

References

Product Information
Components	Neural Stem Cell Basal Medium (Cat.# SCM003), 500 mL bFGF, 10 ug
Quality Level	MQ100

Applications
Application	The Rat Neural Stem Cell Expansion Medium is provided as a two-component system that is convenient & easy to use.
Key Applications	Stem Cell Culture

Biological Information
Media Form	Liquid
Stem Cell Type	Neural Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Each lot of medium is tested for the optimal cell growth, proliferation and differentiation of mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Part number SCM003, the Neural Stem Cell Basal Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month. FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.

Storage and Shipping Information

Storage Conditions

Part number SCM003, the Neural Stem Cell Basal Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.

FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.

Packaging Information
Material Size	1 kit

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
SCM009	04053252663123

Documentation

Rat Neural Stem Cell Expansion Medium Certificates of Analysis

Title	Lot Number
RAT NEURAL STEM CELL EXPANSION MEDIUM	2470941
RAT NEURAL STEM CELL EXPANSION MEDIUM	2989616
RAT NEURAL STEM CELL EXPANSION MEDIUM	3116476
RAT NEURAL STEM CELL EXPANSION MEDIUM	2485885
RAT NEURAL STEM CELL EXPANSION MEDIUM - 2140014	2140014
RAT NEURAL STEM CELL EXPANSION MEDIUM - 2390506	2390506
RAT NEURAL STEM CELL EXPANSION MEDIUM - 2441937	2441937
RAT NEURAL STEM CELL EXPANSION MEDIUM - 2450113	2450113
RAT NEURAL STEM CELL EXPANSION MEDIUM - 1959580	1959580
RAT NEURAL STEM CELL EXPANSION MEDIUM - 2227150	2227150

Related Products & Applications

Kit Component

Catalogue Number

Description

SCM003

Neural Stem Cell Basal Medium

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