Alginate Lyase

Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.

Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .

Alginate Lyase has been used:

• in the pre-processing of enzyme
• to de-encapsulate cells
• to digest Pseudomonas exopolysaccharide to facilitate electroporation

Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.

Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.

Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.

One unit will produce an increase the A_235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer