03-0163-00
SMC® Human TNFα High Sensitivity Immunoassay Kit
Used for ultrasensitive quantification of Human TNFα in human serum and plasma
Synonym(s):
TNF-a immunoassay kit, TNF-alpha Single Molecule Counting Kit, TNF-alpha immunoassay kit, TNF-alpha ultra-sensitive immunoassay, Tumor necrosis factor α
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About This Item
Product Name
SMC® Human TNFα High Sensitivity Immunoassay Kit,
description
Used for ultrasensitive quantification of Human TNFα in human serum and plasma
product line
SMC®
usage
kit sufficient for 96 wells
species reactivity
human
technique(s)
single molecule counting: suitable
input
plasma (K2 EDTA)
serum
UniProt accession no.
compatibility
for use with FemtoQuest™ and SMCxPRO® instruments
shipped in
wet ice
storage temp.
2-8°C
General description
Application
Features and Benefits
- Ultra-Sensitive Detection: Utilizing SMC®technology, this assay detects TNFα at femtogram-level concentrations, enabling precise quantification in human plasma and serum—even in low-expression conditions.
- Familiar Workflow, Elevated Performance: Follows a standard sandwich immunoassay protocol with a unique elution step and digital readout, delivering high signal-to-noise ratios and low background interference.
- Expert Scientific Support: Backed by Field Application Scientists and technical support teams experienced in academic, pharma, CRO, and regulated lab environments.
- Automation-Ready Workflow: Compatible with liquid handling systems for hands-free operation, reducing variability and increasing throughput.
Packaging
Analysis Note
- Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
- Standard Curve Range: 50-50,000 fg/mL
- Intra-assay CV: <15%
- Inter-assay CV: <20%
- Linearity: Serum: 113% Plasma: 105%
- Recovery: Serum: 95% Plasma: 83%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
Other Notes
Legal Information
Disclaimer
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Danger
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 03-0163-00 | 04054839591730 |