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About This Item
UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in Sf21 cells
biological source
human
Quality Segment
recombinant
expressed in Sf21 cells
mol wt
α-subunit Mw 49 kDa (heterotetramer; α2β2), β subunit Mw 53 kDa (heterotetramer; α2β2)
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
General description
Product Source: Expressed in Sf21 insect cells.
Recombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag.
Biochem/physiol Actions
Protein Target: CK2
Packaging
2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.
Physical form
20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
Sequential chromatography using heparin Sepharose™ and Mono Q anion exchange resin
Preparation Note
1 year at -20°C.
Analysis Note
Routinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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