454578
Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)
lyophilized, clone R1JHL, Calbiochem®
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
R1JHL, monoclonal
Species reactivity:
rat
Application:
—
Citations:
-
biological source
mouse
Quality Segment
antibody form
culture supernatant
antibody product type
primary antibodies
clone
R1JHL, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Immunoblotting (1:300, colorimetric)
Immunoprecipitation (3 µg/200 µg lysate))
isotype
IgG
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Mouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.
Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.
This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.
Immunogen
Rat
a recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
Application
Immunoblotting (1:300, colorimetric)
Immunoprecipitation (3 µg/200 µg lysate)
Immunoprecipitation (3 µg/200 µg lysate)
Physical form
Lyophilized from cell culture supernatant.
Preparation Note
Reconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Rat cortex tissue extracts
Rat cortex tissue extracts
Other Notes
Does not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci.14, 3180
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.
Ishii, T., et al. 1993. J. Biol. Chem.268, 2836.
Monyer, H., et al. 1992. Science256, 1217.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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