Human iPSC Derived AFE Progenitors

The anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the lung, esophagus, salivary glands, thymus, parathyroid and thyroid. Our cryopreserved anterior foregut endoderm (AFE) progenitor cells were derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550) in chemically defined/serum-free culture conditions. The AFE cells are double positive for Sox2 (A,C; Sigma AB5603A4; 1:50), Pax9 (B, C; Abcam ab28538; 1:200) and approximately 20-30% are double positive for EpCAM (D, F; Sigma MAB4444; 1:500) and Pax9 (E, F).

The human iPSC derived AFE progenitors are a component of the 3dGRO^® Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the <italic>in vivo</italic> branching airway and early alveolar structures. The 3dGRO^® Human Lung Organoid Culture System includes (all sold separately):

• Definitive Endoderm Induction Medium (SCM302)
• Anterior Foregut Endoderm Induction Medium I (SCM305)
• Anterior Foregut Endoderm Induction Medium II (SCM306)
• 3dGRO^® Lung Organoid Branching Medium (SCM307)
• 3dGRO^® Lung Organoid Maturation Medium (SCM308)
• Human iPSC Derived AFE Progenitors (SCC301)

Research Category:

• Cell Culture
• Stem Cell Research

3X10⁶ cell/vial

Human iPSC Derived AFE Progenitors should be stored in liquid nitrogen.

3dGRO^® is a trademark of Merck KGaA, Darmstadt, Germany.
3dGRO^® organoids were derived utilizing HUB Organoid Technology.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact organoids@milliporesigma.com.

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.