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D8537

Dulbecco’s Phosphate Buffered Saline

liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DPBS

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32129211
NACRES:
NA.75
MDL number:
MFCD00131855
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Product Name

Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture

Quality Level

300

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: no (Dextro)
sodium pyruvate: no

shipped in

ambient

storage temp.

room temp

General description

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.

Application

Dulbecco′s Phosphate Buffered Saline has been used:

  • for the dilution of fresh red blood cells to study the trapping of red blood cell aggregate.
  • to wash the wells of a microtiter plate.
  • to wash the cells before resuspending them in lysis buffer for DNA isolation.
  • as a component of embryo holding medium for vitrification of embryos.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000427783
0000423729
0000423720
0000423708
0000423708

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Protocols

유도만능줄기세포 배양 프로토콜

iPS 세포 해동, 증식, 냉동, 특성화를 포함한 인간 유도만능줄기세포(iPSC)를 위한 줄기세포 배양의 단계별 프로토콜.

Cryopreservation of Cell Lines

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

Subculture of Adherent Cell Lines

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

View All Protocols

Articles

MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

3D 오가노이드 배양: 새로운 발달 및 질병 체외 모델

조직 및 줄기세포 유래 3D 뇌, 장, 소화관, 폐 및 암 종양 오가노이드 모델을 생성하는 오가노이드 배양 제품.

Arturo Levican et al.
Applied and environmental microbiology, 79(16), 4951-4957 (2013-06-19)
The genus Arcobacter is composed of 17 species which have been isolated from various sources. Of particular interest are A. butzleri, A. cryaerophilus, and A. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes
Elliptical optical tweezers for trapping a red blood cell aggregate
Kauppila AJ
Photonics Letters of Poland, 3(3), 95-97 (2011)
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
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