F4523
Fast Red TR/Naphthol AS-MX
SIGMAFAST™, fluorogenic, tablet
Synonym(s):
4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets
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About This Item
Product Name
SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 10 mL
Quality Level
form
tablet
storage temp.
−20°C
Application
Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets has been used in fluorescent in situ hybridization for the analysis of gene expression in neurons and synapses.
Preparation Note
Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2
Storage Class
4.1B - Flammable solid hazardous materials
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Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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