H7140
Hybridization Solution
for Northern and Southern blotting, liquid (2x concentrate)
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
Quality Level
form
liquid (2x concentrate)
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
General description
Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.
Application
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.
Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.
Preparation Note
Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Smooth muscle-specific expression of SV40 large TAg induces SMC proliferation causing adaptive arterial remodeling
Sindermann JR, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 283(6), H2714-H2724 (2002)
Sambrook, J., et al.
Molecular Devices, 9-9 (1989)
Silicon nanonets for biological sensing applications with enhanced optical detection ability
Serre P, et al.
Biosensors And Bioelectronics, 68, 336-342 (2015)