다음 MAP메이트™는 통합될 수 없습니다: -다른 분석 완충용액이 필요한 MAP메이트™. -인산 특이성 및 총 MAP메이트™ 조합, 예: 총 GSK3β 및 GSK3β(Ser 9). -PanTyr 및 자리 특이성 MAP메이트™, 예: Phospho-EGF 수용체 및 phospho-STAT1(Tyr701). -단일 표적(Akt, STAT3)를 위한 1개 이상의 1 phospho-MAP메이트™. - GAPDH 및 β-Tubulin은 panTyr를 포함하는 키트 또는 MAP메이트™와 통합될 수 없습니다.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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List
이 제품은 즐겨찾기에 저장되었습니다.
종
패널 유형
선택하신 키트
수량
카탈로그 번호
주문 설명
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96-Well Plate
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카탈로그 번호
주문 설명
포장 단위
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다른 시약 추가 (MAP메이트 사용을 위해 완충용액과 검출 키트가 필요함)
수량
카탈로그 번호
주문 설명
포장 단위
기재 가격
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
공간 절약 옵션 다수의 키트를 구매하시는 고객은 고용량 저장을 위해 키트 포장을 제거하고 비닐백에 담긴 멀티플레스 분석 구성품을 받아 저장 공간을 절약하도록 선택할 수 있습니다.
이 제품은 즐겨찾기에 저장되었습니다.
해당 제품은 고객님의 카트에 추가되었습니다.
이제 다른 키트를 사용자 지정하거나, 사전 혼합된 키트를 선택하거나, 결재하거나 또는 주문 도구를 종료할 수 있습니다.
Amnis® imaging flow cytometry platforms discriminate cells in flow objectively and statistically based on their appearance. This capability can therefore be used for any experiment that results in changes to the cell’s image.
FRET using the ImageStream®X for detection of protein-protein interactions
In fluorescence microscopy, light at one wavelength is absorbed by a fluorophore and emitted at a longer wavelength. FRET (Fluorescence or Forster Resonance Energy Transfer) can occur when a second fluorophore is in very close contact such that it can accept the energy from the first fluorophore and emit the light at a longer wavelength. The efficiency of the transfer is extremely sensitive to the separation distance between the fluorophores; therefore, when emission is detected at the longer wavelength, the conclusion is that the fluorophores were within approximately 10 nm of each other. When two fluorophores (a donor and acceptor pair) are used to label two different proteins, the close proximity of the two proteins are inferred by the measurement of the FRET from the donor to the acceptor fluorophore. The combination of high speed image acquisition and automated quantitative image analysis with FRET on the ImageStream®X allows measurement of the spatial location of the protein interaction within the cell or between cell conjugates even for rare subpopulations. Distinguishing intracellular location of FRET vs. location of the proteins when not exhibiting FRET behavior offers a major advancement in understanding signaling pathways.
In this experiment receptor 1 is labeled with PE (donor) and receptor 2 is labeled with AF647 (acceptor). Cells were stimulated or not-stimulated and images collected with 488nm laser excitation. The graph shows that FRET to the AF647 fluorophore can be detected in the stimulated sample.
Poster:
Technical Information:
Amnis® imaging flow cytometry platforms discriminate cells in flow objectively and statistically based on their appearance. This capability can therefore be used for any experiment that results in changes to the cell’s image.