다음 MAP메이트™는 통합될 수 없습니다: -다른 분석 완충용액이 필요한 MAP메이트™. -인산 특이성 및 총 MAP메이트™ 조합, 예: 총 GSK3β 및 GSK3β(Ser 9). -PanTyr 및 자리 특이성 MAP메이트™, 예: Phospho-EGF 수용체 및 phospho-STAT1(Tyr701). -단일 표적(Akt, STAT3)를 위한 1개 이상의 1 phospho-MAP메이트™. - GAPDH 및 β-Tubulin은 panTyr를 포함하는 키트 또는 MAP메이트™와 통합될 수 없습니다.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
공간 절약 옵션 다수의 키트를 구매하시는 고객은 고용량 저장을 위해 키트 포장을 제거하고 비닐백에 담긴 멀티플레스 분석 구성품을 받아 저장 공간을 절약하도록 선택할 수 있습니다.
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이제 다른 키트를 사용자 지정하거나, 사전 혼합된 키트를 선택하거나, 결재하거나 또는 주문 도구를 종료할 수 있습니다.
Classical biochemical techniques are often employed to measure location and co-location of molecules, tasks ideally suited for Amnis® imaging flow cytometry system. Examples include molecular translocation of transcription factors from the cytoplasm to the nucleus, trafficking of molecules to sub-cellular organelles, co-localization of proteins on, in, or between cells, just to name a few. The Amnis® imaging flow cytometry's ability to obtain statistically robust per-cell measurements of probe location and co-localization within highly heterogenous samples provides significant advances that complement and extend traditional biochemical research.
Measurement of NFκB translocation in transgenic T cells which are in contact with antigen presenting cells (APC) and specific peptide. Specific T cell:APC conjugates are identified and translocation of NFκB from the cytoplasm to the nucleus specifically within the T cells is measured in the presence (shown) or absence of peptide. See the application note for more details.
Co-Localization of an Antibody-Drug Conjugate to Endosomes or Lysosomes
In this experiment we use the capabilities of the Imagestream®x system to measure the transit of a fluorescently labeled antibody-drug conjugate (ADC) through the cellular endocytic pathway. The high spatial resolution afforded by the Imagestream®x system allowed accurate measurement of ADC co-localization to endosomes and lysosomes. See the application note for more details.
FRET Using the Imagestream®x for Detection of Protein-Protein Interactions
In fluorescence microscopy, light at one wavelength is absorbed by a fluorophore and emitted at a longer wavelength. FRET (Fluorescence or Forster Resonance Energy Transfer) can occur when a second fluorophore is in very close contact such that it can accept the energy from the first fluorophore and emit the light at an even longer wavelength. The efficiency of the transfer is extremely sensitive to the separation distance between the fluorophores and therefore when the emission is detected at the longer wavelength the conclusion is that the fluorophores were within approximately 10 nanometers of each other. When two fluorophores (a donor and acceptor pair) are used to label two different proteins, the close proximity of the two proteins are inferred by the measurement of the FRET from the donor to the acceptor fluorophore. The combination of high speed image acquisition and automated quantitative image analysis with FRET on the Imagestream®x allows measurement of the spatial location of the protein interaction within the cell or between cell conjugates even for rare subpopulations. Distinguishing intracelluar location of FRET vs. location of the proteins when not exhibiting FRET behavior offers a major advancement in understanding signaling pathways.
In this experiment receptor 1 is labeled with PE (donor) and receptor 2 is labeled with AF647 (acceptor). Cells were stimulated or not-stimulated and images collected with 488nm laser excitation. The graph shows that FRET to the AF647 fluorophore can be detected in the stimulated sample.
Classical biochemical techniques are often employed to measure location and co-location of molecules, tasks ideally suited for Amnis® imaging flow cytometry system. Examples include molecular translocation of transcription factors from the cytoplasm to the nucleus, trafficking of molecules to sub-cellular organelles, co-localization of proteins on, in, or between cells, just to name a few. The Amnis® imaging flow cytometry's ability to obtain statistically robust per-cell measurements of probe location and co-localization within highly heterogenous samples provides significant advances that complement and extend traditional biochemical research.
