171539 AMPK (α2/β1/γ1), His•Tag®, Human, Recombinant,S. frugiperda

171539
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      카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
      171539-5UGCN
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          Plastic ampoule 5 μg
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          Description
          OverviewFull length, recombinant, human AMPK consisting of subunits α2/β1/γ1, each fused at the C-terminus to a His•Tag® sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
          Catalogue Number171539
          Brand Family Calbiochem®
          SynonymsAMP Kinase (α2/β1/γ1)
          Application Data
          The activity of AMPK (α2/β1/γ1) was measured as outlined in the Recommended Reaction Conditions.
          References
          ReferencesForetz, M., et al. 2005. Diabetes 54, 1331.
          Viollet, B., et al. 2003. Biochem. Soc. Trans. 31, 216.
          Product Information
          Unit of DefinitionKinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
          Quality LevelMQ100
          Applications
          Biological Information
          Purity≥75% by SDS-PAGE
          Specific Activity≥270 nmol/min/mg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          AMPK (α2/β1/γ1), His•Tag®, Human, Recombinant,S. frugiperda MSDS

          타이틀

          물질안전보건자료(MSDS) 

          AMPK (α2/β1/γ1), His•Tag®, Human, Recombinant,S. frugiperda Certificates of Analysis

          TitleLot Number
          171539

          References

          참고문헌 보기
          Foretz, M., et al. 2005. Diabetes 54, 1331.
          Viollet, B., et al. 2003. Biochem. Soc. Trans. 31, 216.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-September-2008 JSW
          SynonymsAMP Kinase (α2/β1/γ1)
          Application Data
          The activity of AMPK (α2/β1/γ1) was measured as outlined in the Recommended Reaction Conditions.
          DescriptionFull length, recombinant, human AMPK consisting of subunits α2/β1/γ1, each fused at the C-terminus to a His•Tag® sequence and co-expressed in S. frugiperda insect cells using a baculovirus expression system. AMPK is involved in sensing energy levels in the cell that result from changes in AMP levels. AMPK is an upstream regulator of mTOR activation. The α-subunit contains a kinase domain, the β-subunit contains a glycogen binding domain, and the γ-subunit contains the regulatory AMP binding domain. The active complex is useful in the study of kinase activity regulation and inhibitor screening.
          FormLiquid
          FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 250 µM DTT, 100 µM EGTA, 100 µM EDTA, 100 µM PMSF, 25% glycerol, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Recommended reaction conditions
          Kinase assay conditions: Dilute purified AMPK in kinase dilution buffer (5 mM MOPS, pH 7.2, 5 mM MgCl2, 2.5 mM β-glycerophosphate, 1 mM EGTA, 400 µM EDTA, 50 ng/µl BSA, 5% glycerol; add DTT to 25 µM just prior to use) to the desired concentration. Mix 10 µl diluted enzyme, 5 µl SAMStide substrate (HMRSAMSGLHLVKRR) (1 mg/ml stock), and 5 µl 500 µM AMP and incubate for 5 min at 30°C. Add 5 µl ATP mixture [(150 µl of 10 mM ATP, 100 µl of 1 mCi/100µl [32P]ATP, and 5.75 ml kinase assay buffer (25 mM MOPS, pH 7.2, 25 mM MgCL2, 12.5 mM β-glycerophosphate, 5 mM EGTA, 2 mM EDTA with 250 µM DTT just prior to use)] and incubate for 15 min at 30°C. Terminate the reaction by spotting 20 µl of the reaction mixture onto a phosphocellulose P81 paper, dry, and wash several times with 1% phosphoric acid prior to counting in the presence of scintillation fluid.
          Purity≥75% by SDS-PAGE
          Specific activity≥270 nmol/min/mg protein
          Unit definitionKinase activity was measured as the molar amount of phosphate incorporated into SAMS peptide substrate (HMRSAMSGLHLVKRR) at 30°C using a final concentration of 50 µM ATP.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesForetz, M., et al. 2005. Diabetes 54, 1331.
          Viollet, B., et al. 2003. Biochem. Soc. Trans. 31, 216.