178611 Anti-Aquaporin 1 (243-261) Rabbit pAb

178611
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개요

Replacement Information

주요 사양표

Species ReactivityHostAntibody Type
H, R Rb Polyclonal Antibody

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178611-1STCN
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      Description
      OverviewRecognizes aquaporin-1 in red blood cells. Supplied with AQP1243-261 blocking peptide (immunogen).
      Catalogue Number178611
      Brand Family Calbiochem®
      SynonymsAnti-AQP1, Anti-CHIP28
      Application Data
      Detection of rat aquaporin 1 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with the control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 1 (243-261) Rabbit pAb (Cat. No. 179611) (1:400). Detection: chemiluminescence.
      References
      ReferencesKing, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
      Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
      Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.
      Product Information
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, pH 7.4 and 40 µg blocking peptide lyophilized from PBS.
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
      Frozen Sections (see comments)
      Application CommentsThe epitope is unique for AQP1 and is not present in other known related proteins. It is identical in human, rodent, and cattle, and only slightly different in frog. Recognition of AQP1 can be fully inhibited with the soluble AQP1243-261 blocking peptide. For use as a negative control, preincubate 1 µg blocking peptide with 1 µg antibody for 1 h at room temperature. This antibody is also reported to work with frozen sections. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (KVWTSGQVEEYDLDADDIN) corresponding to amino acids 243-261 of human AQP1, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDissolve the antibody in 50 µl deionized water to make a 1 mg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of stock solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Dissolve the peptide in 100 µl deionized water and store in aliquots at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Aquaporin 1 (243-261) Rabbit pAb Certificates of Analysis

      TitleLot Number
      178611

      References

      참고문헌 보기
      King, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
      Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
      Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-June-2007 RFH
      SynonymsAnti-AQP1, Anti-CHIP28
      ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
      Frozen Sections (see comments)
      Application Data
      Detection of rat aquaporin 1 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with the control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 1 (243-261) Rabbit pAb (Cat. No. 179611) (1:400). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the aquaporin-1 protein. Supplied with AQP1243-261 blocking peptide (immunogen).
      BackgroundAQP1 is a 269 amino acid transmembrane protein belonging to the aquaporin family. AQP1 forms the major water transports pathway of red cells and renal proximal tubules and thin descending limb of Henle, but not collecting duct.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (KVWTSGQVEEYDLDADDIN) corresponding to amino acids 243-261 of human AQP1, conjugated to KLH
      IsotypeIgG
      Specieshuman, rat
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, pH 7.4 and 40 µg blocking peptide lyophilized from PBS.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsThe epitope is unique for AQP1 and is not present in other known related proteins. It is identical in human, rodent, and cattle, and only slightly different in frog. Recognition of AQP1 can be fully inhibited with the soluble AQP1243-261 blocking peptide. For use as a negative control, preincubate 1 µg blocking peptide with 1 µg antibody for 1 h at room temperature. This antibody is also reported to work with frozen sections. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsDissolve the antibody in 50 µl deionized water to make a 1 mg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of stock solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Dissolve the peptide in 100 µl deionized water and store in aliquots at -20°C.
      Toxicity Standard Handling
      ReferencesKing, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
      Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
      Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.