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Merck

ABC339

Anti-BFL-1 Antibody

from rabbit, purified by affinity chromatography

동의어(들):

Bcl-2-related protein A1, Bcl-2-like protein 5, Bcl2-L-5, Hemopoietic-specific early response protein, Protein BFL-1, Protein GRS, BFL-1

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Uniprot accession no.:
Conjugate:
unconjugated
Application:
IF, IHC, WB
Citations:
-
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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

human (immunogen homology)

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BCL2A1(597)

General description

Protein BFL-1, also known as protein GRS, Bcl-2-like protein 5 (Bcl2-L-5), and Bcl-2-related protein A1 (BCL2A1), is a member of the Bcl-2 family. BFL-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. BFL-1 is expressed in peripheral blood, spleen, bone marrow, lung, small intestine, and testis.
~39 kDa observed. Uniprot describes two isoforms at ~19 kDa and ~20 kDa

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human BFL-1.

Application

Anti-BFL-1 Antibody is a highly specific rabbit polyclonal antibody, that targets Bcl-2-related protein A1 & has been tested in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2 µg/mL from a representative lot detected BFL-1 in mouse kidney tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected BFL-1 in mouse kidney cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

This antibody recognizes the N-terminus of BFL-1.

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse kidney tissue lysate
Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected BFL-1 in 15 µg of mouse kidney tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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