219361 Anti-Cathepsin D Rabbit pAb

219361
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      개요

      Replacement Information

      주요 사양표

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

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      219361-1MLCN
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          Plastic ampoule 1 ml
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          Description
          OverviewRecognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D in breast carcinoma cell line.
          Catalogue Number219361
          Brand Family Calbiochem®
          Application Data
          Detection of human cathepsin D by immunoblotting. Samples: Low molecular weight markers (lanes 5a and 5b), cathepsin D protein (0.075 µg) (lanes 1 and 9), cathepsin D protein (0.15 µg) (lanes 2 and 8), and cathepsin D protein (0.3 µg) (lanes 3 and 7), and liver extracts (lanes 4 and 6). With primary antibody dilution 1:4000 (lanes 1-5a) and 1:2000 (lanes 5b-9). Primary antibody: Anti-Cathepsin D Rabbit pAb (Cat. No. 219361) (1:4000). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS.
          Positive controlHuman liver tissue
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoelectrophoresis
          Application NotesELISA (≥1:1000)
          Immunoelectrophoresis (see comments)
          Application CommentsMonospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenpurified human liver cathepsin D
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Cathepsin D Rabbit pAb MSDS

          타이틀

          물질안전보건자료(MSDS) 

          Anti-Cathepsin D Rabbit pAb Certificates of Analysis

          TitleLot Number
          219361

          Brochure

          Title
          Cathepsins and Related Products Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-July-2007 RFH
          ApplicationELISA (≥1:1000)
          Immunoelectrophoresis (see comments)
          Application Data
          Detection of human cathepsin D by immunoblotting. Samples: Low molecular weight markers (lanes 5a and 5b), cathepsin D protein (0.075 µg) (lanes 1 and 9), cathepsin D protein (0.15 µg) (lanes 2 and 8), and cathepsin D protein (0.3 µg) (lanes 3 and 7), and liver extracts (lanes 4 and 6). With primary antibody dilution 1:4000 (lanes 1-5a) and 1:2000 (lanes 5b-9). Primary antibody: Anti-Cathepsin D Rabbit pAb (Cat. No. 219361) (1:4000). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~50 kDa latent and the ~30 kDa active forms of cathepsin D.
          HostRabbit
          Immunogen speciesHuman
          Immunogenpurified human liver cathepsin D
          IsotypeIgG
          Specieshuman
          Positive controlHuman liver tissue
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsMonospecific for cathepsin D as determined by a single arc by immunoelectrophoresis against crude live extract and purified cathepsin D. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Toxicity Standard Handling