콘텐츠로 건너뛰기
Merck

AB144P

Anti-Choline Acetyltransferase Antibody

CHEMICON®, goat polyclonal

동의어(들):

Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

주요 문서

모든 문서 보기
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의

제품 이름

Anti-Choline Acetyltransferase Antibody, Chemicon®, from goat

biological source

goat

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

NCBI accession no.

NM_020549.3

UniProt accession no.

P28329

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

chicken ... Chat(395314)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

Analysis Note

Control
Human placenta lysates, rat forebrain/rostral hypothalamus.
Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

Application

Anti-Choline Acetyltransferase Antibody has been used in:
  • immunohistochemical staining
  • western blotting
  • immunocytochemistry

Immunohistochemistry:
A previous lot of this antibody was used at 1:100.

Optimal working dilutions and protocols must be determined by end user.

Biochem/physiol Actions

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.
Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

70/74 kDa
Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.

Immunogen

Human placental enzyme.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Research Category:
Neuroscience

Research Sub Category:
Neurotransmitters & Receptors

Neuronal & Glial Markers

Physical form

Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Plasticity of motor network and function in the absence of corticospinal projection
Han Q, et al.
Experimental Neurology, 194-208 (2015)
The human choline acetyltransferase gene encodes two proteins
Grosman DD, et al.
Journal of Neurochemistry (1995)
Blood pressure regulation by CD4+lymphocytes expressing choline acetyltransferase
Olofsson PS, et al.
Nature Biotechnology, 34(10), 1066-1071 (2016)
Javier Bernácer et al.
PloS one, 2(11), e1174-e1174 (2007-12-15)
The striatum (caudate nucleus, CN, and putamen, Put) is a group of subcortical nuclei involved in planning and executing voluntary movements as well as in cognitive processes. Its neuronal composition includes projection neurons, which connect the striatum with other structures
Choline acetyltransferase structure reveals distribution of mutations that cause motor disorders
Cai Y, et al.
The Embo Journal, 2047-2047 (2004)
모든 관련된 서류 보기

문서

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Antibody Generation | Monoclonal vs Polyclonal Antibodies

Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.

Antibody Basics

구조, 클래스, 면역글로불린의 정상 범위에 관한 기술 정보 등 항체 작업의 기본 원칙에 대해 알아보십시오.

Antibody Basics

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

관련 콘텐츠

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

SNAP i.d. 2.0 System Brochure

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

국제 무역 품목 번호

SKUGTIN
AB144P08436037123283
AG22004053252274930
AB144P-200UL04053252268984
AB144P-1ML04053252468797

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.