biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CRIM1(51232)
Immunogen
Recombinant protein from the cytoplasmic tail of mouse Crim1.
Application
Anti-Cysteine-rich Motor Neuron 1 Antibody detects level of Cysteine-rich Motor Neuron 1 & has been published & validated for use in IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
Western blot: 1:1,000 using ECL. The antibody reacts with proteins of ~120 kDa. Preferred gel percentage is 7.5%. Suggested blocking buffer is PBS containing 5% non-fat milk. Suggested dilution buffer is PBS containing 1% non-fat milk.
Immunoprecipitation: 5 μL to 1 mL of cell extract. Preferred tissue/cell lysis buffer is RIPA. Suggested incubation time is 2-4 hours. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by the end user.
Immunoprecipitation: 5 μL to 1 mL of cell extract. Preferred tissue/cell lysis buffer is RIPA. Suggested incubation time is 2-4 hours. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Crim1
SUBCELLULAR LOCALIZATION: Intracellular and Membrane associated.
SUBCELLULAR LOCALIZATION: Intracellular and Membrane associated.
Physical form
Rabbit serum. Liquid.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
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