Anti-GRASP65 Antibody

Evaluated by Western Blotting in human alzheimer′s brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GRASP65 in 10 µg of human alzheimer′s brain tissue lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT (Joshi, G., et al. (2013). PNAS. 1-10).
Immunocytochemistry Analysis: A representative lot detected GRASP65 in NRK cells (Tang, D., et al. (2012). Biology Open. 1204-1214).
Immunocytochemistry Analysis: A representative lot detected GRASP65 in CHO cells transfected with WT APP and PS1 or APPswe/PS1ΔE9 mutants, cultured primary hippocampal neurons, and WT CHO cells (Joshi, G., et al. (2013). PNAS. 1-10).
Western Blotting Analysis: A representative lot detected GRASP65 in APPswe/PS1ΔE9 cells stably expressing GFP-tagged GRASP65 WT, cells stably expressing GFP-tagged GRASP65 WT, and mouse brain (Joshi, G., et al. (2013). PNAS. 1-10).

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GST-tagged Recombinant protein corresponding to rat GRASP65.

~65 kDa observed

Rabbit polyclonal serum containing 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.