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Merck

MAB1285FX

Anti-HLA-B27,Cln HLA-ABC-m3 FITC (mouse monoclonal)

clone HLA-ABC-m3, Chemicon®, from mouse

동의어(들):

Anti-AS, Anti-HLAB

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702

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제품 이름

Anti-HLA-B27,Cln HLA-ABC-m3 FITC (mouse monoclonal), clone HLA-ABC-m3, Chemicon®, from mouse

antibody form

purified antibody

biological source

mouse

conjugate

FITC conjugate

antibody product type

primary antibodies

clone

HLA-ABC-m3, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable

isotype

IgG2a

NCBI accession no.

NM_005514.5

UniProt accession no.

P01889

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100
300

Gene Information

human ... HLA-B(3106)

관련 카테고리

Application

Detect HLA-B27 using this Anti-HLA-B27 Antibody, clone HLA-ABC-m3, FITC conjugated validated for use in FC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

Flow cytometry and immunofluorescence 1:15 to 1:60

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

Biochem/physiol Actions

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7 (9.5x107M-).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

BIOCHEMISTRY: pI = 8.0
The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

Immunogen

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.

Physical form

Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in PBS 0.01M, Sodium Azide 0.1%, pH 7.1, 0.2% BSA. The characteristics of each lot are tested by flow cytometry.

Preparation Note

Store at 2 to 8°C. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

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국제 무역 품목 번호

SKUGTIN
MAB1285FX04053252617539

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