Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor^™ 488 conjugated

Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through binding with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Compared to fibronectin, collagen, or vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Recently, Laminin-5 is attracting attention as a substrate for carcinoma cells that may stimulate migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, et al., 2002, Lohi, et al., 2001).

Amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein.

Epitope: amino acids 382-608

Immunocytochemistry: 2 μg/mL
Flow cytometry
Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Laminin-5 Antibody, clone D4B5, Alexa Fluor 488 conjugated is validated for use in FC, IC for the detection of Laminin-5.

Monoclonal antibody MAB19562 was developed against amino acid residues 382-608 of human laminin gamma2 chain (III domain) expressed as a GST fusion protein. The antibody recognizes the 150 kDa (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.

Purified immunoglobulin conjugated to Alexa Fluor^™ 488. Liquid in PBS containing 1% BSA, 0.05% Tween-20 and 0.05% sodium azide.

Maintain at 2°-8°C for up to 12 months from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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