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CB1044 Anti-OCLN Mouse mAb (1G7)

This Anti-OCLN Mouse mAb (1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of OCLN.

Anti-OCLN Mouse mAb (1G7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Occludin

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CB1044
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개요

Replacement Information

주요 사양표

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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CB1044-100UGCN
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      		 100 μg
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      Description
      OverviewRecognizes the ~55-60 kDa OCLN protein in HL-60 and HeLa cells.
      Catalogue NumberCB1044
      Brand Family Calbiochem®
      SynonymsAnti-Occludin
      Application Data
      Detection of human OCLN by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human OCLN by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHL-60 cells, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
      Clone1G7
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      CB1044-100UGCN 04055977228403

      Documentation

      Anti-OCLN Mouse mAb (1G7) MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Occludin
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human OCLN by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human OCLN by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~55-60 kDa OCLN protein.
      BackgroundOCLN is an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
      Clone1G7
      IsotypeIgG₁
      Specieshuman
      Positive controlHL-60 cells, HeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review