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MABS270 Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3

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MABS270
100 µL  
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      개요

      Replacement Information

      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS270
      DescriptionAnti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3
      Alternate Names
      • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform
      • PP2A B subunit isoform B'-epsilon

      • PP2A B subunit isoform B56-epsilon
      • PP2A B subunit isoform PR61-epsilon
      • PP2A B subunit isoform R5-epsilon
      Background InformationProtein phosphatase 2A (PP2A) is a family of ubiquitous heterotrimeric proteins that catalyze the removal of phosphate groups from serine and threonine residues. The PP2A enzymes contain core structural (A) and catalytic (C) subunits and variable regulatory B subunits. The B subunits regulate their catalytic activity, as well as dictate substrate specificity and subcellular localization. Recent studies have suggested that B56 epsilon subunits regulate p53-mediated apoptotic pathways, and can induce or suppress apoptosis depending on the cellular context. PP2A enzymes are also regulated by posttranslational modifications and endogenous inhibitors.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3 is validated for use in Western Blotting, ICC, Immunoprecipitation for the detection of PP2A reg. subunit B56 epsilon (PPP2R5E).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesWestern Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of U2OS cell lysate and mouse brain tissue lysate.

      Immunocytochemistry Analysis: A representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in U2OS cells. (Max F. Perutz Laboratories, Medical University of Vienna, Austria).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous PP2A reg. subunit B56 epsilon (PPP2R5E) in a panel of U2OS cell lysates expressing the B56 alpha, beta, gamma, delta, and epsilon regulatory subunit of PP2A (Max F. Perutz Laboratories, Medical University of Vienna, Austria).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human PP2A reg. subunit B56 epsilon (PPP2R5E).
      Clone5A5-1F3
      HostMouse
      SpecificityThis antibody is specific for the B56 regulatory subunit of Protein phosphatase 2A (PP2A).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PPR2R5E
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~50 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluted by Western Blot in NIH/3T3 cell lysate.

      Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABS270 04053252599934

      Documentation

      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3 MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3 Certificates of Analysis

      TitleLot Number
      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) 2478025
      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E), clone 5A5-1F3 - 3457076 3457076
      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E), clone 5A5-1F3 - 3792006 3792006
      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E), clone 5A5-1F3 - 4259201 4259201
      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E), clone 5A5-1F3 - Q2055254 Q2055254
      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E), clone 5A5-1F3 -2695336 2695336
      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E), clone 5A5-1F3 -2765143 2765143
      Anti-PP2A reg. subunit B56 epsilon (PPP2R5E), clone 5A5-1F3 Monoclonal Antibody 3021982

      References

      Reference overviewPub Med ID
      Evidence toward a dual phosphatase mechanism that restricts Aurora A (Thr-295) phosphorylation during the early embryonic cell cycle.
      Kang, Q; Srividhya, J; Ipe, J; Pomerening, JR
      The Journal of biological chemistry  289  17480-96  2014

      요약 표시
      24825897 24825897

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      카테고리

      Life Science Research > Antibodies and Assays > Primary Antibodies