Anti-Pras40 Antibody, Clone 73P21

40 kDa

PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.

Recombinant human PRAS40 (full-length) expressed in E. coli.

Anti-Pras40 Antibody, Clone 73P21 is an antibody against Pras40 for use in IP & WB.

Immunoblot Analysis: A 01-1.0 μg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
PI3K, Akt, & mTOR Signaling

Recognizes PRAS40.

This antibody does not cross react with mouse.

Affinity purified

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.1% sodium azide.

Stable for 1 years at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
RIPA lysates of HeLa cells.

Routinely evaluated by western blot.

Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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