Anti-Tryptophan hydroxylase 1/TPH1 Antibody

Tryptophan hydroxylase 1/TPH1, also known as Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, and encoded by the gene TPH1/TPH/TPRH/TRPH, is an important biosynthetic enzyme needed for serotonin synthesis from L-trytophan. TPH1 is responsible for the addition of the -OH grouip to 5-hydroxytryptophan which is the initial and rate limiting step in the synthesis of serotonin. Serotonin in a neurotransmitter and has an antidepressant effect on the brain. The activity of TPH1 is also modulated by phosphorylation Protein Kinase A is responsible for the phosphorylation of TPH1 and helps to modulate its activity in neurons. TPH1 is mostly expressed in tissues that express serotonin such as the brain, pineal gland, and CNS but also can be found in skin and intestinal tissues.

~51 kDa observed

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of rat Tryptophan hydroxylase 1/TPH1.

Immunohistochemistry Analysis: A 1:50-500 dilution from a representative lot detected Tryptophan hydroxylase 1/TPH1 in mouse pancreas and mouse colon tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Tryptophan hydroxylase 1/TPH1 in mouse cerebellum and mouse cerebral cortex tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Tryptophan hydroxylase 1/TPH1 antibody is validated for use in western blotting & IHC (Paraffin) for the detection of Tryptophan hydroxylase 1/TPH1.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in P815 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Tryptophan hydroxylase 1/TPH1 in 10 µg of P815 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB15570

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