Anti-monoubiquitin H2A Antibody (Lys119)

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The ubiquitination of Histones plays essential roles in the regulation of DNA related processes.

~23 kDa observed. Uniprot describes one isoform at 14 kDa; however, this protein may be observed at ~23 kDa due to monoubiquitination.

Branch peptide corresponding to human monoubiquitin H2A (Lys119).

Detect Histone H2A using this rabbit polyclonal antibody, Anti-monoubiquitin H2A Antibody (Lys119) validated for use in western blotting, ChIP, IP and ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blotting Analysis: A representative lot from an independent laboratory detected monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Affinity purified

Purified rabbit polyclonal in buffer containing 90 mM Tris-glycine pH8, 50 mM Nacl, and 10% glycerol with 0.05% sodium azide

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
HeLa acid extract

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected monoubiquitin H2A (Lys119) in 10 µg of HeLa acid extract.

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