biological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5F10, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr669)
Gene Information
human ... EGFR(1956)
General description
180 kDa
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.
Application
Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
Recognizes EGFR when phosphorylated at Threonine 669.
Physical form
100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Format: Purified
Thiophilic adsoption and size exclusion chromatography
Preparation Note
6 months at -20°C from date of shipment
Analysis Note
Control
Includes PMA and pervanadate treated A431 cell lysate as a positive control
Includes PMA and pervanadate treated A431 cell lysate as a positive control
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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10 - Combustible liquids
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