biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
12A3, monoclonal
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr845)
Gene Information
human ... EGFR(1956)
mouse ... Egfr(13649)
General description
180 kDa
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Tyr 845 in human EGFR.
Application
Detect phospho-EGFR (Tyr845) using this Anti-phospho-EGFR (Tyr845) Antibody, clone 12A3 validated for use in ELISA, IC, IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
Recognizes phosphorylated EGFR on Tyrosine 845.
Physical form
100 µg of mouse monoclonal IgG1 lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose
Format: Purified
Subsequent thiophilic adsorption and size exclusion chromatography
Preparation Note
2 years at -20°C from date of shipment
Analysis Note
Control
Includes EGF treated Hep2G cell lysate as a positive control
Includes EGF treated Hep2G cell lysate as a positive control
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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