218701 Casein Kinase II, Human, Recombinant, E. coli

218701
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      카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
      218701-10KUCN
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          Plastic ampoule 10 ku
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          Description
          OverviewRecombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits (M.W. 44 kDa) and regulatory β-subunits (M.W. 26 kDa) in an α2β2-tetramer. This material corresponds to one of several forms of native CKII. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies.
          Note: 1 KU = 1000 units.
          Catalogue Number218701
          Brand Family Calbiochem®
          SynonymsrhCKII
          References
          ReferencesYamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
          Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
          Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.
          Product Information
          Activity≥500,000 units/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.
          FormLiquid
          FormulationIn 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON® X-100 Detergent, pH 7.5.
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Casein Kinase II, Human, Recombinant, E. coli MSDS

          타이틀

          물질안전보건자료(MSDS) 

          Casein Kinase II, Human, Recombinant, E. coli Certificates of Analysis

          TitleLot Number
          218701

          References

          참고문헌 보기
          Yamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
          Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
          Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision18-November-2008 JSW
          SynonymsrhCKII
          DescriptionRecombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits (M.W. 44 kDa) and regulatory β-subunits (M.W. 26 kDa) in an α2β2- tetramer. This material is one of several forms of native CK-II. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies. The following substrates have been characterized by CK-II both in vitro and in vivo: nucleolin, RNA polymerase, p53, and SV 40 large T antigen.
          FormLiquid
          FormulationIn 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON® X-100 Detergent, pH 7.5.
          Recommended reaction conditions20 mM Tris-HCl, pH 7.5, 50 mM KCl, 10 mM MgCl2, and 200 μM ATP (200 μCi/μmol). GTP can be used as a phosphoryl donor in place of ATP. Incubate at 30°C. Note: optimal incubation times and enzyme concentrations must be determined empirically for each substrate.
          Activity≥500,000 units/ml
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesYamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
          Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
          Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.