234374 Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli

234374
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234374-10UGCN
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      Plastic ampoule 10 μg
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      Description
      OverviewRecombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells.
      Catalogue Number234374
      Brand Family Calbiochem®
      SynonymsrmGM-CSF
      References
      ReferencesAnaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
      Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
      Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
      Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
      Gough, N.M., et al. 1985. EMBO J. 4, 645.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.
      Quality LevelMQ100
      Applications
      ApplicationGranulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli, is a pleiotropic cytokine that can stimulate the proliferation, maturation, and function of hematopoietic cells.
      Biological Information
      Biological activityED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3
      Purity>97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1.0 EU/µg GM-CSF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Granulocyte Macrophage Colony-Stimulating Factor, Mouse, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      234374

      References

      참고문헌 보기
      Anaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
      Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
      Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
      Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
      Gough, N.M., et al. 1985. EMBO J. 4, 645.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-April-2008 RFH
      SynonymsrmGM-CSF
      DescriptionRecombinant, mouse granulocyte macrophage colony-stimulating factor expressed in E. coli. Pleiotropic cytokine that stimulates the proliferation, maturation, and function of hematopoietic cells. Reported to improve the response rate to antibiotic therapy in cancer subjects. Activates JAK2 in a variety of cells. Target cells include macrophages, granulocytes, and eosinophils. GM-CSF is produced by many cell types, including endothelial cells, fibroblasts, and T-lymphocytes. It can be extracted from most tissues in concentrations exceeding that of serum levels. While many cells produce GM-CSF constitutively, levels of this lymphokine increase rapidly in response to endotoxin, phorbol esters, and certain antigens.
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS containing 50 µg BSA/µg GM-CSF.
      Purity>97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1.0 EU/µg GM-CSF
      Biological activityED₅₀ = 100-300 pg/ml as measured in a cell proliferation assay using a factor-dependent cell line, DA-3
      SolubilityReconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 3 months at 4°C.
      Toxicity Standard Handling
      Merck USA index14, 4538
      ReferencesAnaissie, E.J., et al. 1996. Am. J. Med. 100, 15.
      Watanabe, S., et al. 1996. J. Biol. Chem. 271, 12681.
      Moore, M.A. 1991. Annu. Rev. Immunol. 9, 159.
      Nicola, N.A. 1989. Annu. Rev. Biochem. 58, 45.
      Gough, N.M., et al. 1985. EMBO J. 4, 645.