수량	카탈로그 번호	주문 설명	포장 단위	기재 가격
			96-Well Plate

수량	카탈로그 번호	주문 설명	포장 단위	기재 가격
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

507858 Sigma-AldrichPANSORBIN^® Cells

PANSORBIN^® Cells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet
Citations

Synonyms: Staphylococcus aureus Cells

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개요

Replacement Information

가격 및 재고여부

카탈로그 번호	재고 정보	패킹	포장 단위	가격(VAT 별도)	수량
507858-10GMCN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Glass bottle	10 gm	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가
507858-1GMCN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Glass bottle	1 gm	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가
507858-50GMCN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Glass bottle	50 gm	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가
507858-5GMCN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Plastic ampoule	5 gm	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가

Description
Overview	PANSORBIN^® Cells are heat-killed, formalin-fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lyphocytes and for immunoprecipitation.
Catalogue Number	507858
Brand Family	Calbiochem®
Synonyms	Staphylococcus aureus Cells

References
References	Kierszenbaum, F., et al. 1991. Immunology 74, 317. Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569. Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368. Murakami, H., et al. 1988. Biochem. J. 256, 917. Kessler, S.W. 1975. J. Immunol. 115, 1617.

Product Information
Activity	≥2.0 mg human IgG/100 mg cells
Form	Homogeneous, milky suspension
Formulation	Supplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.
HS Code	3002 59 00
Quality Level	MQ100

Applications
Key Applications	Immunoprecipitation

Biological Information

Physicochemical Information
Contaminants	Viable cells: ≤2500 cells/ml

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
카탈로그 번호	GTIN
507858-10GMCN	04055977272505
507858-1GMCN	04055977272499
507858-50GMCN	04055977271942
507858-5GMCN	04055977271935

Documentation

PANSORBIN^® Cells MSDS

타이틀
물질안전보건자료(MSDS)

PANSORBIN^® Cells Certificates of Analysis

Title	Lot Number
507858	로트(lot) 번호를 입력하십시오.

References

참고문헌 보기
Kierszenbaum, F., et al. 1991. Immunology 74, 317. Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569. Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368. Murakami, H., et al. 1988. Biochem. J. 256, 917. Kessler, S.W. 1975. J. Immunol. 115, 1617.

Citations

타이틀

Kira Steigerwald, et al. (2005) The APC tumor suppressor promotes transcription-independent apoptosis in vitro. Molecular Cancer Research 3, 78-89.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	06-May-2010 JSW
Synonyms	Staphylococcus aureus Cells
Description	Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high affinity interaction of protein A with the Fc domain of IgG. PANSORBIN^® cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.
Form	Homogeneous, milky suspension
Formulation	Supplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.
Recommended reaction conditions	The procedures used for performing immunoprecipitations using PANSORBIN^® cells are somewhat variable, but this protocol can serve as a general guideline. PANSORBIN^® cells work best when the antibody is human (IgG₁, IgG₂, IgG₄), rabbit IgG (all isotypes), or mouse (IgG_2a, IgG_2b, IgG₃). Prewashing: 1. Transfer desired amount of PANSORBIN^® cells to a microfuge tube (typically 10-30 µl of a 10% PANSORBIN^® cell suspension per immunoprecipitation). 2. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 3. Aspirate the supernatant. 4. Resuspend to the original volume or in 1.0 ml of cold lysis buffer, whichever is greater. Mix gently. 5. Repeat wash (steps 2-4). 6. Resuspend the final pellet to the original volume (from step 1) in lysis buffer. 7. If kept sterile, washed PANSORBIN^® cells are stable at 4°C. Pre-Clearing (Optional): Pre-clearing with PANSORBIN^® cells prior to immunoprecipitation removes material that binds nonspecifically, reducing the background. 1. Add 10-30 µl of washed PANSORBIN^® cells to the lysate prior to incubation with the primary antibody. 2. Incubate for 1 h on ice, mixing occasionally. 3. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 4. Transfer the supernatant to another microfuge tube and discard the pellet. Alternatively, the lysate can be pre-cleared with PANSORBIN^® cells which have been pre-bound to nonspecific antiserum (see Secondary Antibody pre-binding procedure). Primary Antibody: 1. Add the required amount of primary antibody to the lysate. 2. Incubate for at least 1 h on ice, mixing occasionally. 3. If a secondary antibody step is not required or desired, proceed to the PANSORBIN^® Step described below. Secondary Antibody: In some cases, a secondary antibody will be necessary. For example, if the primary antibody is mouse IgG₁, it is best to use a rabbit anti-mouse IgG as a secondary antibody. If desired, the secondary antibody can be pre-bound to the PANSORBIN^® cells to eliminate the loss of antibody-antigen complexes which have not bound to the protein A. 1. Incubate the desired amount of secondary antibody with prewashed PANSORBIN^® cells for 45-60 min on ice. 2. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 3. Aspirate the supernatant and wash the lysis buffer. 4. Resuspend in lysis buffer to a volume equal to the original volume of PANSORBIN^® cells which were added. Alternatively, the unbound secondary antibody and PANSORBIN^® cells can be incubated with the sample sequentially, following primary antibody incubation. PANSORBIN^® Step: 1. Add at least 10X the amount of PANSORBIN^® cells (alone or bound to secondary antibody) needed to precipitate the primary (or secondary) antibody. PANSORBIN^® cells bind about 2 mg of human IgG/ml. Typically, add 10-30 µl of PANSORBIN^® cells per immunoprecipitation. This quantity gives a visible pellet. 2. Incubate for 1-2 h on ice, mixing regularly. 3. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 4. Wash PANSORBIN^® pellet 2-3 times with lysis buffer. 5. If samples are to be loaded onto a polyacrylamide gel, the PANSORBIN^® pellet can be resuspended in sample buffer, boiled for 10 min, centrifuged quickly (30 s in a microfuge at 20°C). Load the supernatant onto the gel. If a high background is observed, it may be necessary to pre-clear lysates, prebind the antibody to PANSORBIN^® cells, or use a stronger lysis buffer (e.g. RIPA). This protocol is meant to serve as a guideline and may need to be modified for specific applications.
Contaminants	Viable cells: ≤2500 cells/ml
Activity	≥2.0 mg human IgG/100 mg cells
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Kierszenbaum, F., et al. 1991. Immunology 74, 317. Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569. Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368. Murakami, H., et al. 1988. Biochem. J. 256, 917. Kessler, S.W. 1975. J. Immunol. 115, 1617.
Citation	Kira Steigerwald, et al. (2005) The APC tumor suppressor promotes transcription-independent apoptosis in vitro. Molecular Cancer Research 3, 78-89.

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카테고리

Life Science Research > Protein Detection and Quantification > Immunodetection Support Products > Protein A & Protein G Reagents

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