General description
Complex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K, and untagged, recombinant, murine p85α full length.
The E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
p110α(E542K) MW = 129 kDa, p85α MW = 84 kDa.
Packaging
Also available in 250μg size --call for pricing and availability and reference catalog number 14-791M when ordering the 250μg size.
Also available in 10 μg size (cat#14-791) and in bulk (cat# 14-791-K).
Physical form
50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Preparation Note
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Analysis Note
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.