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21-170 TOPflash (TCF Reporter Plasmid)

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      Replacement Information
      Catalogue Number21-170
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionTOPflash (TCF Reporter Plasmid)
      Product Information
      HS Code2934 99 99
      Quality LevelMQ100
      ApplicationTransfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame.
      Key Applications
      • Transfection
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.
      Gene Symbol
      • TCF1
      • MODY3
      • HNF1
      • HNF-1A
      • TCF-1
      • HNF1A
      • LFB1
      • HNF1a
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20823 # Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
      SIZE: 631 amino acids; 67356 Da
      SUBUNIT: Binds DNA as a dimer.
      DISEASE: SwissProt: P20823 # Defects in HNF1A may predispose to hepatic adenomas [MIM:142330]. Hepatic adenomas are benign tumors at risk of malignant transformation. Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. & Defects in HNF1A are the cause of maturity onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY [MIM:606391] is a form of diabetes characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion. The clinical phenotype of MODY3 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications. & Defects in HNF1A are a cause of susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100].
      SIMILARITY: SwissProt: P20823 ## Belongs to the HNF1 homeobox family. & Contains 1 homeobox DNA-binding domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size5 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      카탈로그 번호 GTIN
      21-170 04053252276354


      TOPflash (TCF Reporter Plasmid) MSDS



      TOPflash (TCF Reporter Plasmid) Certificates of Analysis

      TitleLot Number
      TOPflash (TCF Reporter Plasmid) (TK-luciferase reporter) 5.5kb 3012082
      TOPflash (TCF Reporter Plasmid) - 18908 18908
      TOPflash (TCF Reporter Plasmid) - 19491 19491
      TOPflash (TCF Reporter Plasmid) - 19963 19963
      TOPflash (TCF Reporter Plasmid) - 20756 20756
      TOPflash (TCF Reporter Plasmid) - 23206 23206
      TOPflash (TCF Reporter Plasmid) - 26217 26217
      TOPflash (TCF Reporter Plasmid) - 27752 27752
      TOPflash (TCF Reporter Plasmid) - 3853786 3853786
      TOPflash (TCF Reporter Plasmid) - DAM1560242 DAM1560242


      Reference overviewApplicationPub Med ID
      Histone deacetylase 7 controls endothelial cell growth through modulation of beta-catenin.
      Margariti, Andriana, et al.
      Circ. Res., 106: 1202-11 (2010)  2010

      요약 표시
      20224040 20224040
      Characterization and functional analysis of the murine Frat2 gene
      van Amerongen, R., et al
      J Biol Chem, 279:26967-74 (2004)  2004

      15073180 15073180
      Murine Frizzled-1 behaves as an antagonist of the canonical Wnt/beta-catenin signaling
      Roman-Roman, S., et al
      J Biol Chem, 279:5725-33 (2004)  2004

      14627707 14627707
      Adenomatous Polyposis Coli (APC)-independent Regulation of {beta}-Catenin Degradation via a Retinoid X Receptor-mediated Pathway
      Xiao, J. H., et al
      J Biol Chem, 278:29954-29962 (2003)  2003

      Transfection12771132 12771132
      The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF.
      Ishitani, T, et al.
      Nature, 399: 798-802 (1999)  1999

      요약 표시
      10391247 10391247
      Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse.
      Korinek, V, et al.
      Mol. Cell. Biol., 18: 1248-56 (1998)  1998

      요약 표시
      9488439 9488439
      The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.
      Roose, J, et al.
      Nature, 395: 608-12 (1998)  1998

      요약 표시
      9783587 9783587
      Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF
      van de Wetering, M, et al
      Cell, 88:789-99 (1997)  1997

      9118222 9118222
      Species differences in kidney necrosis and DNA damage, distribution and glutathione-dependent metabolism of 1,2-dibromo-3-chloropropane (DBCP).
      E J Søderlund,M Låg,J A Holme,G Brunborg,J G Omichinski,J E Dahl,S D Nelson,E Dybing
      Pharmacology & toxicology  66  1990

      요약 표시
      2371234 2371234