주요 문서

모든 문서 보기

AB144P

Anti-Choline Acetyltransferase Antibody

Name: Anti-Choline Acetyltransferase Antibody
Brand: MM

CHEMICON^®, goat polyclonal

동의어(들):

Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Clone:

polyclonal

Species reactivity:

human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken

Application:

ICC, IHC (p), WB

Citations:

1732

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

속성

제품 이름

Anti-Choline Acetyltransferase Antibody, Chemicon^®, from goat

biological source

goat

Quality Segment

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken

manufacturer/tradename

Chemicon^®

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

NCBI accession no.

NM_020549.3

UniProt accession no.

P28329

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

chicken ... Chat(395314)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)

설명

General description

70/74 kDa

Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.

Immunogen

Human placental enzyme.

Application

Anti-Choline Acetyltransferase Antibody has been used in:

immunohistochemical staining
western blotting
immunocytochemistry

Immunohistochemistry:
A previous lot of this antibody was used at 1:100.

Optimal working dilutions and protocols must be determined by end user.

Biochem/physiol Actions

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.

Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

Physical form

Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

Analysis Note

Control
Human placenta lysates, rat forebrain/rostral hypothalamus.

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Research Category:
Neuroscience

Research Sub Category:
Neurotransmitters & Receptors

Neuronal & Glial Markers

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Still not finding the right product?

Explore all of our products under Anti-Choline Acetyltransferase Antibody

— 또는 —

제품 선택 도구}를 사용하여 선택의 폭을 좁혀보세요.

저장 등급

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c