Anti-NMDAR2A Antibody

180 kDa

NMDA (N-methyl-D-aspartate) receptors are a class of ionotropic glutamate receptors. The NMDAR channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D).

C-Terminal Fusion Protein of rat NMDAR2A, aa 1253-1391.

Epitope: C-terminus

Anti-NMDAR2A Antibody is an antibody against NMDAR2A for use in IH, IP & WB.

Immunoprecipitation:
3 μg of a previous lot was used to (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200 μg of rat brain.

Immunohistochemistry:
A previous lot of this antibody was used at 1:1,000-1:2,000 dilution.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

NMDAR2A. By Western blot it recognizes a 180 kDa band in rat brain membranes. Does not react with NMDAR2B or NMDAR2C. Immunolabeling is blocked by preadsorption of antibody with the immunogen.

ImmunoAffinity Purified

Purified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservative.

Lyophilized material stable for 1 year at -20°C from date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up 6 months.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
NMDAR2A is present in high concentrations in the hippocampus.

Routinely evaluated by Western Blot on Rat Brain Microsomal lysates.

Western Blot Analysis: 1:500 dilution of this lot detected NMDAR2A on 10 μg of Rat Brain Microsomal lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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