Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody

Alpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.

~58 kDa observed

KLH-conjugated linear peptide corresponding to human Alpha-ketoglutarate-dependent dioxygenase FTO.

Immunoprecipitation Analysis: 10 μg from a representative lot immunoprecipitated Alpha-ketoglutarate-dependent dioxygenase FTO from 500 μg of U2OS nuclear extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody is validated for use in western blotting & IP for the detection of Alpha-ketoglutarate-dependent dioxygenase FTO.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in U2OS nuclear extract.

Western Blotting Analysis: 1 μg/mL of this antibody detected Alpha-ketoglutarate-dependent dioxygenase FTO in 10 μg of U2OS nuclear extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.