conjugate
unconjugated
Quality Segment
antibody form
serum
clone
polyclonal
purified by
(unpurified)
species reactivity
human, mouse, rabbit
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunohistochemistry: suitable, western blot: suitable
UniProt accession no.
target post-translational modification
unmodified
General description
41.65 kDa calculated
LPH-conjugated linear peptide corresponding to the signal and propeptide of human Pro-Biglycan.
Application
Western Blotting Analysis: A representative lot detected Pro-Biglycan in BMP1 treated recombinant protein (von Marschall, Z., and Fisher, L.W. (2010). Biochem. Biophys. Res. Commun. 391(13):1374-1378).
Biochem/physiol Actions
This antiserum recognizes only the biglycan preproprotein (368aa) and probiglycan (349aa), but not the mature biglycan (331aa) lacking the N-terminal signal and propeptide sequence. This polyclonal antiserum is also known by LF-104.
Physical form
Rabbit polyclonal antibody serum with 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Immunohistochemistry in human and mouse skin tissue.
Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected Pro-Biglycan in human and mouse skin tissue.
Immunohistochemistry Analysis: A 1:500 dilution of this antibody detected Pro-Biglycan in human and mouse skin tissue.
Other Notes
Human, Mouse. Predicted to react with Monkey, Rat, Dog, Cat, and Horse based on 100% sequence homology.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.