Goat Anti-Human IgG Antibody, Fc, Alkaline Phosphatase conjugate

Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Goat anti-Human IgG Antibody, Fc, Alkaline Phosphatase conjugate detects level of Human IgG & has been published & validated for use in ELISA & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

Western Blot:
1:5,000-1:50,000 dilution can be used.

Optimal working dilutions must be determined by the end user.

Based on immunoelectrophoresis, the antibody reacts with the heavy chain of human IgG but not with the light chains of most human immunoglobulins.. No antibody was detected against normal human IgM or IgA, or against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.

Goat secondary antibody IgG in Lyophilized Buffer containing 0.01 M Tris HCl, 0.25 M NaCl, pH 8.0 with 15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

Unpurified

Stable for 1 year at 2–8°C from date of receipt.
After reconstitution the product is stable for several weeks at 2–8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Routinely evaluated by Enzyme-linked Immunosorbent Assay.

ELISA:
1:5,000-1:50,000 dilution can be used.

Replaces: MABN1055

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.