biological source
sheep
Quality Segment
conjugate
CY5 conjugate
antibody form
purified antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rabbit
technique(s)
immunocytochemistry: suitable
shipped in
wet ice
target post-translational modification
unmodified
General description
Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Immunogen
Rabbit IgG
Application
Immunocytochemistry Analysis: Performance of this secondary antibody is validated (1.25 µg/mL) by indirect fluorescent labeling of H3K27me3 in HUVECs following a rabbit polyclonal parimary antibody (Cat. No. 07-449) in immunocytochemistry application.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
This Sheep Anti-Rabbit IgG Antibody, Cy5 conjugate is validated for use in Immunocytochemistry for the detection of Sheep Rabbit IgG.
Biochem/physiol Actions
This antibody reacts with rabbit IgG and rabbit IgG (Fc). Minimal cross-reactivity detected with Human IgG and Rabbit IgM; little to no cross-reactivity detected with Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.
Physical form
Protein A purified
Purified sheep antibody Conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in NIH/3T3 cells.
Immunocytochemistry Analysis: Performance of this secondary antibody is validated (1.25 µg/mL) by indirect fluorescent labeling of H3K27me3 in NIH/3T3 following a rabbit polyclonal parimary antibody (Cat. No. 07-449) in immunocytochemistry application.
Immunocytochemistry Analysis: Performance of this secondary antibody is validated (1.25 µg/mL) by indirect fluorescent labeling of H3K27me3 in NIH/3T3 following a rabbit polyclonal parimary antibody (Cat. No. 07-449) in immunocytochemistry application.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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