Milli-Mark Anti-CD3 -PECy5 Antibody, clone UCHT1

20 kDa (Campana, 1987)

Human TCR/CD3 is a complex structure on the lymphocyte surface. It consists of the TCR alpha beta or TCR gamma delta heterodimer and the associated CD3 complex. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon. and zeta. Three different dimers, gamma epsilon, delta epsilon, and zeta zeta, constitute the CD3 complex. The MW of CD3epsilon is 20 000 (Campana, 1987). CD3 is first detectable in early thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface (Swerdlow, 1988; Beverley, 1981). The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies (McMichael, 1987). Consistent with the pattern of synthesis of the antigen in normal thymocytes, the earliest site detectable within neoplastic cells is the cell cytoplasm (Swerdlow, 1988).

Human infant thymocytes and lymphocytes from a patient with Sézary disease.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Milli-Mark Anti-CD3 -PECy5 Antibody, clone UCHT1 is validated for use in FC for the detection of CD3.

Antibody recognizes Human CD3 antigen.

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to PE/Cy5 in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Human Lymphocytes

Evaluated by flow cytometry using Human Lymphocytes.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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