MAB1021
Anti-Tumor Necrosis Factor-α Antibody, clone 2C8
clone 2C8, Chemicon®, from mouse
동의어(들):
TNF-alpha
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
2C8, monoclonal
Application:
ELISA, IHC, Neutral
Citations:
7
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
clone
2C8, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, neutralization: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TNF(7124)
Immunogen
Human recombinant tumor necrosis factor α.
Application
Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
Detection of TNF-α in two-site immunoassay.
Immunohistochemistry detected TNF-α.
TNF-α Neutralization Assay
1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.
2. Incubate overnight at 37°C in humidified 5% CO2 incubator.
3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL
4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.
5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.
6. Incubate 24 hours at 37°C, 5% CO2.
7. Score wells for cytotoxicity.
Optimal working dilutions must be determined by the end user.
Detection of TNF-α in two-site immunoassay.
Immunohistochemistry detected TNF-α.
TNF-α Neutralization Assay
1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.
2. Incubate overnight at 37°C in humidified 5% CO2 incubator.
3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL
4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.
5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.
6. Incubate 24 hours at 37°C, 5% CO2.
7. Score wells for cytotoxicity.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Human tumor necrosis factor-a (TNF-a)
No cross-reactivity observed with rat neuronal tissue.
Physical form
Format: Purified
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to one year.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1114
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.