Anti-MMP-13 Antibody, clone VIIIA2

Human recombinant collagenase-3 protein.

Immunohistochemistry (frozen and formalin-fixed/paraffin*): 2-4 μg/mL for 60 minutes at room temperature.

* Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecipation: use protein G (2 μg/mg protein lysate)

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

This Anti-MMP-13 Antibody, clone VIIIA2 is validated for use in IH for the detection of MMP-13.

It recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Shows no cross reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster. Cellular Localization: Cytoplasmic

Format: Purified

Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Control
POSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) human fibrosarcoma HT-1080 cells. Bladder, breast, ovarian carcinomas.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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