Anti-Myocilin (NT) Antibody, clone 7.1

Myocilin (mutated trabecular meshwork-induced glucocorticoid response protein) is encoded by the MYOC gene (also known as GLC1A, GPOA, JOAG, JOAG1, TIGR) in human (Entrez Gene ID 4653). Myocilin is produced as a 504 amino acid glycoprotein containing an N-terminal hydrophobic signal peptide sequence, a coiled-coiled leucine zipper domain, and a C-terminal domain with homology to olfactomedins. Myocilin is commonly identified as a 53/57 kDa doublet by gel electrophoresis, and a 66 kDa form of the protein is also reported. Myocilin is known to undergo intracellular endoproteolytic cleavage between Glu214 and Leu215, resulting in a 20 kDa N-terminal and a 35 kDa C-terminal fragment. Myocilin is identified as a Lingo-1 receptor ligand and MYOC gene mutations are linked to 10% of juvenile open-angle glaucoma cases and 3-4% of those with primary open-angle glaucoma (PMID 24732711 24741044, 24837143). Cat. No. MABN866, clone 7.1 is a mouse monoclonal antibody that recognizes an epitope within the N-terminal fragment (aa33-214) and is demonstrated to be suitable for Western blotting, immuncytochemistry, and immunhistochemistry applications. This clone is reactive toward both human and murine species. (PMID 18674535 & 23979599).

~53, 60 kDa observed

Epitope: N-terminal fragment (aa 33-214)

Recombinant protein corresponding to the N-terminal fragment (aa 33-214) of human Myocilin.

Anti-Myocilin Antibody, clone 7.1 is an antibody against Myocilin for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A representative lot detected Myocilin in human trabecular tissue (Ezzat, M.K., et al. (2008). Exp. Eye Res. 87(4):376-384).
Western Blotting Analysis: A representative lot detected Myocilin in human trabecular tissue (Ezzat, M.K., et al. (2008). Exp. Eye Res. 87(4):376-384).
Western Blotting Analysis: A representative lot detected Myocilin in aqueous humor (Zhao, Y., et al. (2013). Mol. Cell Biol. 33(21):4225-4240).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in mouse eye tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Myocilin in 10 µg of mouse eye tissue lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.