Anti-PL Scramblase 1 Antibody, clone 9A7

Phospholipid scramblase 1 (UniProt Q9JJ00; also known as Ca(2+)-dependent phospholipid scramblase 1, Nor1, PL scramblase 1, Tra1, Transplantability-associated protein 1) is encoded by the Plscr1 (also known as Tra1b, Tras1) gene (Gene ID 22038) in murine species. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid “scrambling” between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37 kDa erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity.

~38 kDa observed. Two additional bands shown correspond to heavy and light chains of endogenous IgG from the mouse thymus tissue.

Epitope: Cytoplasmic domain

Recombinant protein corresponding to the cytoplasmic domain of mouse PL Scramblase 1.

Anti-PL Scramblase 1 Antibody, clone 9A7 is an antibody against PL Scramblase 1 Antibody for use in Western Blotting.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western Blotting Analysis: A representative lot detected PL Scramblase 1 in mouse lung homogenates. Clone 9A7 also detected LPS treatment-induced PL Scramblase 1 upregulation in liver using total tissue homogenates, as well as cytosolic and nuclear extracts from LPS-treated and untreated mice (Lu, B., et al. (2007). Biochim. Biophys. Acta. 1771(9):1177-1185).

Clone 9A7 reacts with murine PL Scramblase 1, and does not cross-reacts with murine PL Scramblase 2/3/4 or human PL Scramblase 1.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in mouse thymus tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PL Scramblase 1 in 10 µg of mouse thymus tissue lysate.

Concentration: Please refer to lot specific datasheet.

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