MAB360
Anti-Glial Fibrillary Acidic Protein Antibody
CHEMICON®, mouse monoclonal, GA5
동의어(들):
GFAP
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
제품 이름
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5, ascites fluid, clone GA5, Chemicon®
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GA5, monoclonal
species reactivity
rat, human, mouse, rabbit, chicken, pig, bovine
packaging
antibody small pack of 25 μL
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GFAP(2670)
관련 카테고리
Physical form
Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Application
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
Biochem/physiol Actions
Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Reactivity with other species has not been determined.
General description
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
~ 51 kDa
Immunogen
Purified GFAP from porcine spinal cord.
Other Notes
Replaces: 04-1031; 04-1062
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저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Lateral fluid percussion: model of traumatic brain injury in mice.
Alder, J; Fujioka, W; Lifshitz, J; Crockett, DP; Thakker-Varia, S
Journal of Visualized Experiments null
Y Jiang et al.
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Pirlo, RK; Sweeney, AJ; Ringeisen, BR; Kindy, M; Gao, BZ
Biomicrofluidics null
Jan Gunnar Sørbø et al.
FEBS letters, 581(25), 4884-4890 (2007-09-28)
Aquaporin-4 (AQP4) has been reported to be upregulated post-partum in pregnancy and in early lung development. Several technical challenges exist in measuring AQP4 protein levels, among them sensitivity to detergent solubilization, sample heating and gel composition. Here we have optimized
프로토콜
Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MAB360 | 08436037123559 |
| MAB360-25UL | 04054839337673 |
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