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Merck

56748

Imidazole

BioUltra, Molecular Biology, ≥99.5% (GC)

동의어(들):

1,3-Diaza-2,4-cyclopentadiene, Glyoxaline

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제품정보 (DICE 배송 시 비용 별도)

실험식(Hill 표기법):
C3H4N2
CAS 번호:
Molecular Weight:
68.08
NACRES:
NA.25
PubChem Substance ID:
eCl@ss:
39161001
UNSPSC Code:
12352005
EC Number:
206-019-2
MDL number:
Beilstein/REAXYS Number:
103853
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grade

Molecular Biology

Quality Level

vapor pressure

<1 mmHg ( 20 °C)

product line

BioUltra

assay

≥99.5% (GC)

form

flakes, powder or crystals

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.2% water

ign. residue (900 °C)

≤0.05%

pH

9.5-11.0 (25 °C, 0.1 M in H2O)

pKa (25 °C)

6.95

bp

256 °C (lit.)

mp

88-91 °C (lit.)

solubility

H2O: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg, sulfate (SO42-): ≤50 mg/kg

SMILES string

c1c[nH]cn1

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: 0.10, λ: 280 nm Amax: 0.10

InChI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

InChI key

RAXXELZNTBOGNW-UHFFFAOYSA-N

Application

Excellent for buffers in the range of pH 6.2-7.8
Imidazole can be used to prepare buffers in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

Other Notes

Review: As RNase model


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Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C

저장 등급

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

flash_point_f

293.0 °F - closed cup

flash_point_c

145 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges



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관련 콘텐츠


Review: As RNase model.
R. Breslow
Accounts of Chemical Research, 24, 317-317 (1991)
Aviva Levina et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 19(11), 3609-3619 (2013-01-31)
An anti-metastatic drug, NAMI-A ((ImH)[Ru(III) Cl4 (Im)(dmso)]; Im=imidazole, dmso=S-bound dimethylsulfoxide), and a cytotoxic drug, KP1019 ((IndH)[Ru(III) Cl4 (Ind)2 ]; Ind=indazole), are two Ru-based anticancer drugs in human clinical trials. Their reactivities under biologically relevant conditions, including aqueous buffers, protein solutions
Eleonora Petryayeva et al.
Langmuir : the ACS journal of surfaces and colloids, 29(3), 977-987 (2013-01-10)
Methods have been developed for the solid-phase detection of nucleic acids using mixed films of quantum dots (QDs) and oligonucleotide probes in microtiter plates. Polystyrene microwells were functionalized with multidentate imidazole ligands to immobilize QDs. Oligonucleotide hybridization was transduced using